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Showing 5 results for Azizzadeh

Saber Barkhori-Mehni, Saeed Khanzadi, Mohammad Hashemi, Mohammad Azizzadeh,
Volume 2, Issue 4 (9-2017)

Background: The aim of this study was to investigate antimicrobial effect of Zataria multiflora Boiss essential oil (EO) against six fish spoilage bacteria for evaluation of its potential utilization in the preservation of minimally processed fish products.
Methods: Firstly, GC-MS analysis of the EO was performed to determine its chemical composition. Then, antibacterial effect of the EO in a range of 0.031 to 4 mg/ml was tested against different fish spoilage bacteria such as Aeromonas hydrophila, Pseudomonas aeruginosa, Pseudomonas fluorescens, Shewanella putrefaciens, Escherichia coli and Bacillus subtilis by broth microdilution method to determine minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations.
Results: GC-MS results showed that phenolic components such as carvacrol (51.55%) and thymol (25.49%) were predominant constituents of the EO. Zataria multiflora Boiss EO exhibited strong antimicrobial activity against all tested bacteria. Shewanella Putrefaciens was the most sensitive bacteria with MBC value of 0. 5 mg/ml.
Conclusion: According to the results, this EO could be used as an important natural alternative to prevent bacterial growth in food specially seafood products to preserve them against bacterial spoilage.
Fatemeh Raji, Saeid Khanzadi, Mohammad Hashemi, Mohammad Azizzadeh,
Volume 5, Issue 1 (3-2019)

Background: Active antimicrobial packaging is a novel method for increasing the safety and shelf life of food products. The present study aimed to investigate the inhibitory effects of chitosan coating nano-emulsion incorporated with Zataria multiflora and Bunium persicum essential oils at the concentrations of 0.5% and 1%, respectively on E. coli O157:H7 in vacuum-packed fish samples during 12 days of refrigeration.
Methods: The samples were divided into various groups, including control (no coating), 2% chitosan, 2% chitosan nano-emulsion, and chitosan coating nano-emulsion containing Zataria multiflora and Bunium persicum essential oils at the concentrations of 0.5% and 1%, respectively. The samples were vacuum-packed and stored at refrigeration temperature, and bacterial counting was performed on days zero, one, two, four, six, eight, and 12.
Results: The mean bacterial count had a significant difference between the study groups during 12 days of storage (P < 0.001). The most significant inhibitory effect on the growth of E. coli O157:H7 was observed with nano-emulsion of chitosan containing 1% of Bunium persicum.
Conclusion: According to the results, using the nano-emulsion of chitosan coating with essential oils could effectively decrease the growth of E. coli O157:H7 in food products, especially food especially fish.

Mahzad Hosseini, Abdollah Jamshidi, Mojtaba Raeisi, Mohammad Azizzadeh,
Volume 5, Issue 2 (6-2019)

Background: The Study aimed to investigate the chemical composition, antimicrobial effects, and antioxidant properties of clove and lemon verbena essential oils (EOs).
Methods: The chemical composition of the EOs was identified using gas chromatography/mass spectrometry (GC/MS). In addition, the antibacterial effects of EOs against seven important foodborne bacteria were assessed using the disk-diffusion, agar well-diffusion, and broth microdilution assays. Evaluation of the antioxidant properties of the EOs was carried out using DPPH, β-carotene-linoleic acid bleaching, and reducing power assay.
Results: All the tested bacteria demonstrated susceptibility to EOs, with the highest susceptibility observed in Bacillus cereus to the clove EO in the agar disk-diffusion test. Moreover, Shigella dysenteriae was identified as the most sensitive bacterium to the lemon verbena EO. Salmonella typhimurium was the most resistant bacterium to both EOs. In the agar well-diffusion test, Pseudomonas aeruginosa and S. typhimurium had the lowest sensitivity to the clove and lemon verbena EOs, respectively. Although both EOs exhibited significant antioxidant capacity, the lemon verbena EO showed higher antioxidant activity in all the tests, with the exception of β-carotene-linoleic acid bleaching test.
Conclusion: Clove and lemon verbena EOs could be regarded as potential sources of natural antioxidant and antimicrobial agents in food processing.

Saeid Khanzadi, Asghar Azizian, Mohammad Hashemi, Mohammad Azizzadeh,
Volume 5, Issue 2 (6-2019)

Background: The present study aimed to determine the chemical composition and in-vitro antibacterial activity of Ziziphora clinopodioides essential oil (ZCEO).
Methods: The chemical composition of ZCEO was determined using gas chromatography-mass spectrometry (GC-MS) analysis. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were calculated using the microwell dilution assay to assess the antibacterial effects of ZCEO emulsion and nano-emulsion on E. coli O157:H7.
Results: A yield of 1% (w/w) was identified for ZCEO isolation and pulegone (58.78%), menthone (1.15%), and isomenthone (9.91%) as the key components of ZCEO phytochemicals. The MIC values of the ZCEO emulsion and nano-emulsion were 0.8 and 0.025 mg/ml, respectively, and the MBC values were estimated at 1.6 and 0.05, respectively.
Conclusion: It is recommended that the nano-emulsion of ZCEO be applied as a potential source of natural preservatives in food industries.

Batool Soltaninezhad, Saeid Khanzadi, Mohammad Hashemi, Mohammad Azizzadeh,
Volume 6, Issue 1 (3-2020)

Background: Antimicrobial compounds have numerous applications, and essential oils could be used in edible films to enhance the food shelf life. This study aimed to assess the inhibitory effects of a chitosan/cellulose nanofiber (CNF) film containing the nanoemulsions of Bunium persicum essential oil (NBPEO) and Trachyspermum ammi essential oil (NTEO) on Escherichia coli O157:H7 inoculated in hamburger during storage  (4°C).
Methods: After inoculation, the hamburger samples were classified into three treatment groups of, control, chitosan containing 7.5% CNF (Ch-CNF), and chitosan containing 7.5% CNF enriched with 1.6% NTEO and 0.8% NBPEO (Ch-CNF-NEO). The samples were preserved at the temperature of 4°C, and the bacterial counts were determined on days zero, three, six, nine, and 12. Data analysis was performed using Bonferroni post-hoc test and repeated measures ANOVA.        
Results: The mean E.coli O157:H7 count significantly decreased in the treatments groups compared to the control group. In addition the Ch-CNF-NEO film exerted the most significant inhibitory effects on the growth of E. coli O157: H7 in the hamburger samples.
Conclusion: According to the results, the Ch-CNF-NEO film could effectively reduce the growth of E. coli O157:H7 in hamburger. Therefore, Ch-CNF-NEO film could be used to effectively increase the safety of meat products against E. coli O157: H7.

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